2-Naphthol Impregnation Prior to Steam Explosion Promotes LPMO-Assisted Enzymatic Saccharification of Spruce and Yields High-Purity Lignin

نویسندگان

چکیده

The recent discovery that impregnation with a carbocation scavenger may improve the enzymatic saccharification of steam-exploded softwood has brought softwood-based biorefinery closer to reality. However, nature effect remains unresolved, and its impact on process efficiency product quality in high-dry matter reactions underexplored. Here, we show 2-naphthol enables complete spruce cellulose by lytic polysaccharide monooxygenase (LPMO)-containing Cellic CTec2, but not an LPMO-poor cellulase cocktail (Celluclast), 10% dry industrially feasible enzyme dose reaction time. Importantly, this remarkably high yield correlates increased LPMO activity, which is due ability lignin drive reaction. These findings improves only reducing adsorption inactivation also boosting oxidative depolymerization LPMOs. Pyrolysis lignin-rich residues revealed had little lignin-derived components resulting bio-oil, which, efficient saccharification, showed reduced levels carbohydrate-derived reduce oil storage stability. results bring prospect spruce-based combines biochemical thermochemical conversion routes.

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ژورنال

عنوان ژورنال: ACS Sustainable Chemistry & Engineering

سال: 2022

ISSN: ['2168-0485']

DOI: https://doi.org/10.1021/acssuschemeng.2c00286